import hmac import hashlib import base64 dig = hmac.new(b'1234567890', msg=your_bytes_string, digestmod=hashlib.sha256).digest() base64.b64encode(dig).decode() # py3k-mode 'Nace+U3Az4OhN7tISqgs1vdLBHBEijWcBeCqL5xN9xg='