df.at['Total', 'MyColumn'] = df['MyColumn'].sum()
print (df)
X MyColumn Y Z
0 A 84.0 13.0 69.0
1 B 76.0 77.0 127.0
2 C 28.0 69.0 16.0
3 D 28.0 28.0 31.0
4 E 19.0 20.0 85.0
5 F 84.0 193.0 70.0
Total NaN 319.0 NaN NaN