from pydub import AudioSegment
from pydub.playback import play
# read in audio file and get the two mono tracks
sound_stereo = AudioSegment.from_file(myAudioFile, format="mp3")
sound_monoL = sound_stereo.split_to_mono()[0]
sound_monoR = sound_stereo.split_to_mono()[1]
# Invert phase of the Right audio file
sound_monoR_inv = sound_monoR.invert_phase()
# Merge two L and R_inv files, this cancels out the centers
sound_CentersOut = sound_monoL.overlay(sound_monoR_inv)
# Export merged audio file
fh = sound_CentersOut.export(myAudioFile_CentersOut, format="mp3")