df.iloc[row_index][column_index]
In [3]: sub_df
Out[3]:
A B
2 -0.133653 -0.030854
In [4]: sub_df.iloc[0]
Out[4]:
A -0.133653
B -0.030854
Name: 2, dtype: float64
In [5]: sub_df.iloc[0]['A']
Out[5]: -0.13365288513107493
your_var = df.iloc[row_indexer,column_indexer]